A Study On The Biological Transformation And The Effects Of Acupuncture Of Pressure Signals Of Fibroblast Cells In Vitro Induced By Imitating Needle Stimulation

Objective: By exploring the pressure signal's biological transformation of fibroblast cells induced by mechanical stimulation, to provide possible theoretical and experimental basis for the point principles of "undergoing stimulation, preventing and treating diseases".Methods: Take pressure on fibroblast cells in vitro. Morphological changes were observed, the levels of Prostaglandin E₂ (PGE₂), nitric oxide (NO), interleukin-6 (IL-6) outside the cell were detected, the expresses of IL-6 and the synthesis amount of proteins within the cell were examined. Flow Cytometry (FCM) was used to examine the quantities of DNA, and the changes of proliferation (PI) were calculated, then the correlativity of the proteins and PI were analyzed.Results: â‘ The value of mechanical press used in such experiment did no marked impact on the shape of fibroblast cells. â‘¡The pressure force significantly accelerated the release of PGE₂ and NO. â‘¢The pressure force caused a great increase of the amount of proteins, while nearly did nothing on PI, and the two sides had not notable statistical correlations. â‘£The pressure force obviously promoted both the expresses of intracellular EL-6 and the release of it outside the cell.Conclusions: â‘ The effective stimulation of acupuncture is in fact a kind of mechanical force. The point is made up of many kinds of tissues, but the connective tissues act as the basics. Mechanical press imitating acupuncture could induce themain group cells——fibroblast cells, which constitutes of such basic tissues,transform the pressure signals into biological ones. It indicates that point could directly experience the stimulation by point-related cells except from nerve tissue, and transform mechanical signals into biological ones. While such a transformationis possibly the modem biological principle of point——"undergoing stimulation".â‘¡Most signal molecules simultaneously act as effective molecules. These signal molecules transmit outside the cell and particularly or generally adjust the pathophysiological state of body. Do these extensive biological effects of these signal molecules provide some possible theoretical basis for such biological functions of point as "diseases prevention and treatment".