P～(18) P～(19) KAI1 TIMP-1 And TIMP-2 Research On The Correlation Between Genes And Digest Tumor

Tumour, particularly the digestive malianancy always threatensthe humen's health, and curing tumor illness is always the heat topicsof whole humen society. However, the development of curing tumorillness still slower, though the humen's understanding to the Tumor'growing environment always developing. Gene Treatment has broughtus to a new erea for the disease. With the research development of thehummen gruadually realizing that tumor is gence illness which causedthe abnormity hyperplasia of cell clone. Tumor to cancer is a processof multiple steps that refer to a series of molecule event. These takeplace on the gene change inside of cell, which causes losing control ofcell growing and leads to tumor's occurring. For different type oftumor formation and different period tumor development, it will haveits own dedicated gene change. For the formed cancer formation,cancer cell could have the transfering property only when some geneshave further changing. Most of the cancer took place refer to multiplesteps and multiple genes changing. During the tumor takes place, itcauses the cells'fuction losing when control cancer's growth, whilecancer genes activizing implys the abnormal functions increasing.Therefore, understand what the genes have been changed throughresearch work, and based on which take correspondent measures tocontrol the canceration or basically cure the tumor. When the celllacks of cancer restraining gene, it can correct the cell throughinserting a normal copied gene, so as to stop the cell canceration.While for an over expressed cancer gene, it can interdict the cancergene expressing at the interface of the gene level and cancer gene,through inserting an anti series of cancer gene.p18, p19, KAT1, TIMP-1 and TIMP-2 genes refer to varioustumors occurring, but less publications explain the relation betweenthe genes and tumors growing in hummen's digestive system,particularly P18, P19 genes. The data for the project research here areall based on the living object specimen of clinic patients, and based onwhich, the expression and relations, development and transform of theabove 5 genes in digestive system have been systematically throughstuding of pathology and clinic, and molecule mechanism. Theobjective of the project research is to expore new methods for thefurther clinic application of genes diagnosis and actual therapy fordigestive system tumor.Research Approach Taking living specimen from 34 oesophagus cancer patients(simultaneously taking the normal surrounding tissues from the 34patients for comparison), 22 stomach cancer patients (simultaneouslytaking the normal surrounding tissues from the 22 patients and makingcomparison with 10 cases of gastric ulcer, 9 cases of pathologicalchanges of inflammation of the coat of stomach), 26 large intestinecancer patients (simultaneously taking the normal surrounding tissuesfrom the 26 patients, making comparison with 12 cases ofpathological change of large intestine inflammation.), 16 liver cancerpatients (making comparisons with 16 cases of liver cyst tissues.)through gastroscope/operation. By use of molecule hybridizing atoriginal position, the expression of P18, P19, KAI1, TIMP-1 andTIMP-2 genes of digestric system tumors and their relations amongthe various clinic parameters have been studied from the aspect ofmolecule mechanism Achivements 1,Regarding P18 gene. (1) P18mRNA was showing highexpression in oesophagus cancer patients with positive expression rate76.47%, which has obvious difference feature compared with thenormal tissuse P18mRNA at the surroundings of the oesophaguscancer (P<0.05). Positive expression maily took place in the cellplasm of oesophague, and the expression extent refers to the invasiondepth of the cancer tissues, differentiation extent and whether haslymph transform or not, but has no relations with patient'sex, age andtype of the cancer tissue. (2) P18mRNA has middle expression inlarge intestines cancer with positive rate 22.7%, 34.62%, which hasobvious difference feature of expression compared with the normaltissues at the surroundings (P<0.05). Their expression refers to theinvasion depth of the cancer tissues, whether has lymph transform, buthas nothing to do with differentiation extent. (3) P18mRNA wasshowing higher positive expression rate 87.50% in liver cancer andliver cyst patients, which has obvious difference feature comparedwith the expression of liver cyst patients (P<0.05), and the expressionextent has nothing to do with differentiation extent of cancer tissueand whether has lymph transform or not. 2,Regarding P19mRNA. (1) P19mRNA was showing highexpression in oesophagus cancer patients with positive expression rate32.35%, which has obvious difference feature compared with thenormal tissuse at the surroundings of the oesophagus cancer (P<0.05).Positive expression maily took place in the cancer cells plasm, and theexpression extent refers to the invasion depth of the cancer tissue,differentiation extent and whether has lymph transform or not. (2)P19mRNA expressed in stomach cancer and large intestines cancermainly in cancer cells plasm with positive rate 18.18%, 34.63%,which has obvious difference feature of expression compared with thenormal tissues at the surroundings (P<0.05). The expression extentrefers to the invasion depth of the cancer tissues, whether has lymphtransform or not, but has nothing to do with cancer cellsdifferentiation extent and the patient's sex, age. (3) P19mRNA wasshowing higher positive expression rate 93.75% and 87.50% in livercancer and liver cyst, which has no significant difference between thetwo rates (P>0.05). And the expression extent has nothing to do withdifferentiation extent of liver cancer tissue and whether has lymphtransform or not. 3,Regarding KAI1 gene. (1) KAI1mRNA was showing positiveexpression rate 67.65%, which has no obvious difference featurecompared with the normal tissues (58.82%) at thesurroundings,(P>0.05). The expression extent refers to the invasiondepth of the cancer tissue, differentiation extent and whether haslymph transform or not (negative correlation). (2) KAI1mRNA haslower expression in stomach cancer with positive rate 4.55%, whichhas no obvious difference feature of expression compared with thenormal tissues at the surroundings, the pathological changes ofenteritis (P>0.05). (3) KAI1mRNA was showing positive expressionrate 42.31% in large intestines cancer patient, 46.15% at the normaltissue surrounding the cancer, 50.00% in pathological changes ofenteritis, and which has no obvious difference feature when comparedwith KAT1mRNA positive rate 42.31% in large intestine cancertissues (P>0.05). However, KAI1mRNA expression in large intestinecancer refers to the invasion depth of the cancer tissue, differentiationextent and whether has lymph transform or not (negative correlation).(4) KAI1mRNA has expression rate 62.50% in liver cancer patient,56.25% in liver cyst patient, and the two rates has no obviousdifference (P>0.05). Th expression strength in liver cancer patient hascorrelation with the invasion depth of cancer tissue, differentiationextent and as well as whether lymph has transformed or not (nagotivecorrelation). 4,Regarding TIMP-1. (1) TIMP-11mRNA has expression rate11.76% in oesophagus cancer patient, which has no significantdifference when compared with normal tissue at the surroundings(P>0.05). And the expression has correlation with the invasion depthof cancer tissue, differentiation extent and whether lymph hastransformed or not. (2) TIMP-11mRNA has expression rate 18.18%and 19.23% respectively in stomach cancer and large intestines cancer,and the expession strength in large intestines cancer has correlationwith the invasion depth and whether has lymph transformed ornot(P<0.05), but has nothing to do with the condition of cancer tissuedifferentiation(P>0.05). The expression strength in large intestinescancer patient has correlation with cancer tissue's invasion depth,differentiation conditions and as well as whether the lymph hastransformed or not(P<0.05). TIMP-1mRNA has no expressionshowing in the normal tissues at the surroundings, the pathologicalchanges of gastric ulcer, gastritis and enteritis. And it has significantdifference when compared with stomach cancer tissue and largeintestines cancer tissue (P<0.05). (3) TIMP-1mRNA was showingpositive expression rate 18.75% in stomach cancer patient, but nothingin liver cyst patient. The expression difference is obvious betweenthem. TIMP-1mRNA expression strength in liver cancer tissue hascorrelation with cancer tissue's differentiation status, tissue'histologyand whether has lymph transforming or not. 5,Regarding TIMP-2. (1) TIMP-2mRNA has expression rate50.00% in oesophagus cancer patient, which has significant differencewhen compared with normal tissue (2.94%) at the surroundings. Andthe expression strength has correlation with the invasion depth ofcancer tissue, differentiation extent and whether lymph hastransformed or not. (2) TIMP-2mRNA has expression rate 18.18% and34.62% respectively in stomach cancer and large intestines cancer,which has significant difference when compared with the normaltissue at the surroundings of stomach cancer, gastric ulcer,pathological changes of enteritis, the normal tissue at the surroundingsof large intestines cancer, pathological changes of large intestinesenteritis. (3) TIMP-2mRNA has positive expression rate 37.50% inliver cancer tissue, but nothing shows in liver cyst. So the expressiondifference is obvious between these two. The expression strength ofTIMP-2mRNA in cancer tissue has correlation with differentiationextent of the cancer tissue and whether has lymph transforming or not. Conclusions 1,P18, P19 mRNA has higher positive expression rate in livertumor patient, which can be considered for liver tumor diagnosingsign. Particularly, P18 gene has significant expression difference inliver cancer and liver cyst patients, and has no correlation withdifferentiation extent of cancer tissue and whether has lymphtransforming or not, which could be considered as tumor sign. 2,KAI1mRNA has no significant expression difference amongthe stamoch cancer, the normal tissue at the surroundings, pathologicalchanges of gastric ulcer and enteritis, which matches the case that noliterature report on the issue presently. KAI1mRNA has higherpositive expression rate 67.65%, and which has no significantdifference when compared with the normal tissue (58.82%) at thesurroundings. However, the expression strength has correlation withinvasion depth, differentiation stength of cancer tissue, and as well aswhether has lymph transforming or not (negative correlation), whichnot agree with the real case of literature report on the issue presently. The significance and breakthrough points of the project research P18, P19, KAI1, TIMP-1 and TIMP-2 genes have correlationswith various tumors occurring, however, There are less literaturereport worldwide on the correlations of the tumor of digestive systemavailable, particularly lesser report on P18, P19. Up to now, noliterature published, as the thesis presented here, conducting the studyon the correlations of occurring, developing and transforming of 5types genes from pathology and clinical point of view, and through...