Expression And Clinical Significance Of MMP-9 And Its Inhibitors TIMP-1,TIMP-2 In Non-small Cell Lung Cancer

Objeetive To studay the expression and clinical significance of matrix metalloproteinases-9 and its inhibitors TIMP-1,TIMP-2 in NSCLC.Methods To 113 patients with NSCLC as the experimental group, 20 cases of inflammatory pseudotumor as control group. By using tissue chip technique creating 113 cases NSCLC tissue chip. At the same time the expression of MMP-9 and and its inhibitors TIMP-1,TIMP-2 was detected in 113 cases of NSCLC and 20 cases of inflammatory pseudotumor by immunohistochemical method, and the correlation was analyzed between the expression of these proteins and the clinicopathologic parameter, lymph node metastasis and the prognosis of patients.Results 1. The positive expression rate of MMP-9, TIMP-1 and TIMP-2 in 113 cases of NSCLC tissues were 75.2% (85/113) , 78.8% (89/113) and 67.3% (76/113) respectively, and in 20 cases of inflammatory pseudotumor were 40% (8/20),30% (6/20) and 30% (6/20) respectively. The expression of MMP-9, TIMP-1 and TIMP-2 in NSCLC was significantly higher than in inflammatory pseudotumor. There was statistically significant (P<0.05). 2. The positive expression rate of MMP-9, TIMP-1 and TIMP-2 in 113 cases of NSCLC respectively: (1) male were 76.8%, 81.2% and 68.1%, and female 72.7%, 75.0% and 65.9%. There was no statistically significant (P>0.05). (2) the patients under the age of 60 were 78.0%, 81.4% and 64.4%, and those over the age of 60 were 72.2%, 75.9% and 70.4%. There was no statistically significant (P>0.05). (3) the patients of smoking were 73.1%, 79.1% and 65.7%, and non-smoking were 78.3%, 78.3% and 69.6%. There was no statistically significant (P>0.05). (4) the primary tumor size≤3cm were 54.2%, 62.5% and 58.3%, and the primary tumor lesions>3cm were 80.9%, 83.1% and 69.7%. There was no statistically significant (P>0.05). (5) the pulmonary squamous carcinoma were 66.2%, 78.5% and 70.6%, and adeno-squamous cell carcinoma were 87.5%, 79.2% and 58.3%. There was no statistically significant(P>0.05). (6) high-middle histological grades were 76.6%, 78.1% and 60.9%, and low histological grades were 73.5%, 79.6% and 75.5%. There was no statistically significant(P>0.05). (7) no lymp hnode metastasis were 60.0%, 64.4% and 37.8%, and lymph node metastasis were 85.3%, 88.2% and 86.8%. There was statistically significant(P<0.05). (8) the stage-Ⅰa nd-Ⅱtumours were 66.7%, 89.4% and 58.3%, and the stage-Ⅲa nd-Ⅳtumours were 90.2%, 95.1% and 82.9%. There was statistically significant(P<0.05). (9) the survival rates over 3-year afteroperation were 60.5%, 65.1% and 39.5%, and under 3-year afteroperation were 84.3%, 87.1% and 84.3%. There was statistically significant (P<0.05). 3. The expression of MMP-9,TIMP-1 and TIMP-2 in NSCLC had significantly negative correlation with eaeh other(P<0.05), and The expression of TIMP-1 and TIMP-2 in NSCLC had significantly positive correlation with eaeh other(P<0.05).Conclusions 1. The expression of MMP-9,TIMP-1 and TIMP-2 were significantly higher in NSCLC than inflammatory pseudotumor, which was closely related to malignant biological behavior of NSCLC. 2. These proteins were closely related to lymph node metastasis and clinical TNM stage in NSCLC, which may indicate that they played an important role in tumor invasion and metastasis. 3. There was closely relations between these proteins and postoperative survival of NSCLC patients, and they may be good predictors of NSCLC. 4. These proteins were closely correlated with each other, suggesting that they may have synergistic effect in the development and process of invasion and metastasis in NSCLC. 5. By using tissue microarray technique, it can improve the efficiency of the organization of Pathology, save test materials and reagents, reduce the experimental error, and make more scientific results.