Study Of Nano-gold Immunochromatographic Quantitative Assay Based On Photoelectric Detection And Signal Processing

Immuno-assay is a sensitive detection technique in the field of clinical diagnostics,especially in medical studies and clinical detection. With the development ofimmuno-assay, traditional detection methods in clinical diagnostics have been replacedby many new techniques. One of such technique is the nano-gold immunochro-matographic assay.Nano-gold immunochromatographic assays with uncontaminated, simple singlestep detection, stable reagent, and accelerated analyses have been gained greatattention in clinical diagnostics. Such assay has developed to step in the front of thefield in clinical diagnostics.Currently nano-gold immunochromatographic assay is mainly applied inqualitative and semi-quantitative immuno-detection. Due to the lack of quantitativedetection, the nano-gold immunochromatographic assay is limited in widen applicationin clinical diagnostics. For such reason, it has more practical value to study anano-gold immunochromatographic quantitative assay.The dissertation put forward a new concept and idea through further study ofdetection principle in nano-gold immunochromatographic assay. The concept and ideaare to combine a nano-gold immunochromatographic assay; a technique ofphoto-electric detection based on optic-fiber sensing, and signal processing, especiallyfocused on the study of characteristics of spectrum to achieve a stable and exactquantitative detection in nano-gold immunochro-matographic assay. The developednano-gold immunochromatographic quantitative assay provides to be a new and usefultool in clinical diagnosis.The research works of the dissertation are summarized below:(1) To design a nano-gold immunochromatographic quantitative assay withartificial intelligence through the studying of the principle of nano-goldimmunochromatographic detection and the spectrum signal. While analyzing differentfactors produced in quantitative detection, such as noises of biochemical reaction,optics and electricity, and establishing an idea mathematical expression of thespectrum. (2) To introduce an exchange of wavelet transformation,a model of fuzzycluster recognition and wavelet neural network into the quantitative detection innano-gold immunochromatographic assay. These works are mainly done byacquiring the characteristics of test strip edge, filtering noises, and fitting of curve,etc. Such treatments make it possible from studying to practical application innano-gold immunochromatographic quantitative assay using artificial intelligencesignal processing. The application of nano-gold immunochromatographicquantitative assay in the dissertation has developed for the application of artificialintelligence and signal processing in the field of biomedical science. (3) To transfer the signal of nano-gold immunochromatographic assay to thesignal of spectrum using photoelectric sensor, it which is based on theBeer-Lambert Law and the principle of light compensation. To use photoelectricdetection based on photo-fiber sensor and the design introduced a new artificialintelligence operator -Particle swarm optimization, a stable and exact detection isenhanced in this assay. (4) To study the new concept and idea of the nano-gold immunochromatographicquantitative assay of artificial intelligence, to complete the design of the quantitativedetector based on TMS320LF2407 DSP. The quantitative detector has been tested inbatches of clinical samples and is proven that the concept and idea of the nano-goldimmunochromatographic quantitative assay of artificial intelligence in the dissertationis correct and successful in application. The new concept and idea of artificial intelligence nano-goldimmunochromatographic assay are put forward for the first time in this dissertation.The designed system made it possible in stable and exact quantitative detection in...