Estabolishment Of Transplantated Animal Model Of Human Endometriosis With Immune Reconstruction In Sever Combined Immunodeciency Mice

Objectives: The current medical treatment of endomerriosis ,a common gynecological disease ,is still associated with a high recurrence rate following drug and surgical treatment.To estabolish an appropriate in-vivo model for future research we develop an transplantated animal model of human endometriosis in humanized severe combined immunodeficiency mice.Methods: According to the objectives of the research ,animals were divided into 4 groups:group A nude mice intraperineally injected with the endometrum of patients with uterine myoma,group B SCID mice intraperineally injected with the secretory endometrum of patients with uterine myoma,group C SCID mice intraperineally injected with human peripheral blood lymphocyte for immune reconstruction and group D SCID mice intraperineally injected with human peripheral blood lymphocyte(PBL) and intraperineally injected with the secretory endometrum of patients with uterine myoma after 24 hours of transplantation.Morphology of the ectopic endometrium in animanls 2 weeks and 4 weeks after implantation wan investigated.Human immlunoglobulins in scid mouse serum and peritoneal douching fluid were assayed by ELISA, and hunman lymphocytes in murine spleen wereevaluated by flow cytometry and immunohistologic stainings respectively.Results:The ratio of successful endometrium transplantation was 75%(5/8),100%(10/10),80%(8/10) respectively.The transplantation lesions persisted for up to 28 days revealing a well preserved glandular morphology.The concentration of immlunoglobulins in scid mouse after 2 weeks of transplantation was (2.4910 + 0.8249) ug/ml and (2.2588 + 0.8901) ug/ml in group C and D respectively,and was both significantly greater than that in group B(p<0.01, p<0.05, respectively. The concentration of immlunoglobulins in scid mouse after 4 weeks of transplantation was (6.4713+3.5290) ug/ml and (3.6966+1.5260) ug/ml in group C and D respectively , and was both significantly greater than that in group B(p<0.01, p< 0.05, respectively. The concentration of immlunoglobulins in human-SCID mouse perineal fluid was much greater than that in serum .Part human CD 3 and CD 19 lymphocytes were also detected in human-SCID spleen by cytometry and immunohistologic stainings.Conclusions: THE estabolishment of the animanl model of endometriosis in SCID mouse and in hu-PBL-SCID mouse is successful which can procide an ideal tool for further investigation of the mechanisms and medical therapy of endometriosis.