The Infection Risks Of PERV To SCID Mice Population In Islet Xenotransplantation

Objective:Detection of the PERV subtype in novo Landrace and the infection risks to SCID mice after xenotransplantation; the infection risks of PERV to their population in horizontal transmission and vertical transmission.Methods:Analysed the PERV subtype in SCID mice hepatic tissues; Celiac transplanted novo Landrace islet cells to SCID mice after separation, purification, and cultivation, then killed all the mice afer co-habitation with the not been treated mice and gave birth to young mice, extracted DNA and RNA of some tissues, detected the PERV infection risks to SCID mice, evaluated the infection risks in horizontal transmission and vertical transmission.Result:PERV was integrated in the Landrace genome and it was PERV-A, PERV-B and PERV-C, and the PERV-RNA was positive in liver tissues. PERV-gag sequence was found in SCID mice after celiac islet transplantion, but we did not find PERV-gag sequence in their spouses and their offsprings.Conclusion:Landrace was competent to be suitable donar in PERV infection model. We had establised SCID mice model in PERV infection successfully, but we did not find infection evidence in their spouses and their offsprings.