Amplifications Of TAOS1 And EMS1 Genes In Oral Carcinogenesis

Gene amplification is one of the major mechanisms for activation of oncogenes. Chromosomal band 11q13 is a frequently amplified genomic segment in a large number of human tumors and is thought as a potential biomarker for diagnosis and prognosis. Both TAOS1 and EMS1 reside in the amplified 11q13. Until now, no study has been devoted to focus on the relationship between TAOS1, EMS1 and oral carcinogenesis. Therefore,our study is aimed to investigate the amplifications of TAOS1 and EMS1 genes in the progress of oral carcinogenesis and their clinical significance.By using microdissection method, we obtained normal mucosa, hyperplasia epithelia, mild-dysplasia epithelia, moderate-dysplasia epithelia, severe-dysplasia epithelia and primary OSCC tissue. Then we analyzed the amplifications of TAOS1 and EMS1 genes by using differential display polymerase chain reaction and their relationships with clinicopathological parameters.We observed the following results: (1) TAOS1 or EMS1 amplified indysplasia oral mocusa and OSCC tissue. In some cases, they coamplified;(2)The genetic aberrance of TAOSl was earlier than EMSl in oral carcinogenesis;(3)7]4O57 amplification, EMSl amplification and coamplification correlated with the presence of lymth node metastasis and poorly differenciated histology of OSCC.Now, we could conclude that: (1) TAOSl amplification, EMSl amplification and coamplification could be found in primary OSCC specimens. (2) TAOSl and EMSl amplifications parallel with the progress of oral carcinogenesis, suggesting their potential roles in oral carcinogenesis. TAOSl gene might be involved earlier than EMSl gene in oral carcinogenesis. (3) TAOSl amplification or EMSl amplification is associated with the presence of lymph node metastasis and poorly differenciated histology of cancer, suggesting they are candidate biomarkers for diagnosis and prognosis in OSCC. However, before their routine use in clinical practice, further examination with larger OSCC samples is needed to implement and confirm the previous results.