A Study Of Species Identification Based On Molecular Biology In Forensic Entomology

Objective. The purposes of our work are to establish new methods of species identification in forensic entomology. Methods. Using primer design software, we designed the primer in microsatellite region of chromosome and mitochondrial DNA, respectively. PCR reactions were carried out on ABI9600 thermal cycler and the products of PCR were analyzed by vertical non-denaturing polyacrylamide gel electrophoresis. Sequencing reactions were carried out using one of the PCR primers and the Big Dye Terminator Cycle Sequencing Kit. DNA sequences were obtained on ABI Model 377 DNA Sequencers. Phylogenetic analysis was carried out using the MEGA2.1 and PHYLIP 3.06 software package. Result. 16rsRNA genes on mitochondrial genome were sequenced from six different necrophagous flies from nine regions in China. Phylogenetic trees were constructed on basis of their sequences. Amicrosatellite marker was found to be specific for Musa domestica vicina Maequart. Phylogenetic trees were constructed on basis of allele frequencies of population samples from three regions in China. Conclusion. These genetic markers can be used to identify necrophagous flies in forensic entomolory. Two methods based on different genetic markers were developed for species identification. Our results implicated that these methods based on molecular biology were useful for forensic entomology.