Multiplex Amplification Of Mitochondrial DNA For Species Identification In Forensic Science

Objective: Species identification for biological specimen is one of the main research subjects in forensic biology. Up to now, there are many shortcomings, such as low-sensitivity, short-duration, time-consuming, high-expense, etc. In order to solve these problems, we employed the sequences of appropriate genes in Mitochondrial DNA to develop reliable system for species identification.Methods: Two genes were chosn and primers were designed according to their sequences. The multiplex amplification system of Mitochondrial DNA for species identification was established using two sets of primers, one was universal, and another was human-specific. Following the...