Clinical And Experimental Study Of The Role Of Antibodies To Oxidized Low Density Lipoprotein In Atherosclerosis

We studied the role of antibodies to OX-LDL in the pathological process of atherosclerosis(AS) from three aspect:1.Clinic study:With the technique of ELISA ,we found the level of antibodies in peripheral circulation of the patients in CHD,especially in the ACS,was higher than the control,which had no statistical correlation with the degree of stenosis of coronary.The result of the study meant that the antibodies had the value to be a factor to evaluate the degree of danger in the CHD;2.Animal experiment:With the technique of immunohistochemistry we observed that the IC of IgG cumulated with the progress of AS in aninmal model of AS,and simvastatin could lessen the IC as well as inhibited the progress of plaque.The IC of IgM deposited little in plaque. The result showed that some IC played a role in all stage of the formation of plaque,and simvastatin maybe was an immunomodulator as well as drug for cholesterol lowering;3. Cytology experiment:With the technique of semi-quantitative RT-PCR,we found that there were down regulation of the expression of mRNA of CD36, CD40, MMP-9 in monocyte spured by OX-LDL when intervention with antibodies to OX-LDL.That showed that the close relation between the antibodies to OX-LDL and AS maybe resulted from the protective mechanism of the body.