| AIM: Salusin-6 is a new regulatory peptide relevant to the cardiovascular system and its cardiovascular effects are poorly known. The aim of the present study was to investigate whether salusin-6 can alleviate ischemia-reperfusion injury and clarify its mechanisms. METHODS: The effect of salusin-6 on heart function was observed in isolated rat hearts after ischemia-reperfusion. The effect of salusin-6 on calcium overload induced by hypoxia-reoxygenation or perfusion of Na+ free solution were investigated in isolated rat ventricular myocytes. Furthermore, the effects of salusin-β on INaCa, ICa,L, IKATP, IK1,Ito, Isus and INa were observed in isolated rat ventricular myocytes. RESULTS: 1) Salusin-6 significantly improved post-ischemic myocardial dysfunction in the isolated perfused rat hearts after ischemia and reperfusion injury;2) Salusin-6 alleviated calcium overload of isolated rat ventricular myocytes induced by hypoxia- reoxygenation or perfusion of Na+ free solution;3) Salusin-6 significantly shorten APD and inhibited INaCa and ICa,L and increased Ito, but did not affect IKATP, IK1, Isus and INa. CONCLUSIONS: These results show that salusin-6 can alleviate ischemia-reperfusion injury and improve heart function, which may be mainly or at least partly contribute to the alleviation of calcium overload. Salusin-6 may prevent calcium overload by inhibiting INaCa and ICa,L (decrease calcium influx directly) and increasing Ito and shorten APD (decrease calcium influx indirectly) ,which subsequently alleviates ischemia-reperfusion injury and improve heart function...
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