A Study On Preventing Rats From AD Via RTMS And NSAIDS/AO

Objective: By the methods of ethology, electrophysiology, ultramicropathology and immunohistochemistry, from the view of both Neurology and Neuroinformatics, on micro- and macro-levels of the neural system, the effects of preventing rats from AD were studied via repetitive transcranial magnetic stimulation, non-steroidal anti-inflammatory drug and antioxidant agent.Methods: Altogether 9 groups (12 rats for each group)involved. 3 of 9 groups were repectively administrated VC+VE, Ibuprofen and VC+VE+Ibuprofen as medicine pre-interventions before AD models established;3 of 9 were given low-frequency rTMS, high-frequency rTMS and false rTMS respectively as non-medicine pre-intervention, 1 of 9 as control, 1 of 9 as normal saline group and 1 for AD models directly. In this study, rat models were established by co-injecting Aβ and Ibo into bilateral nucleus basalis of Meynert. Morris water maze test, EEG and long term potentiation (population spikes, PS ) and BDNF level in hippocampus were measured before and after co-injection for AD model. The correlative dimension of EEGs were also estimated. Results:1. AD rat models were successfully estebilshed with severe learning and memory defects by co-injecting A β and Ibo into bilateral nucleus basalis of Meynert.2. All of the established AD rat models in the group without any intervention were verified by its long escape latency, low Dc value of EEG and BDNF level compared with the control.3. The direct AD model group and the false rTMS-given group got worse results inMorris tests, Dc of EEG, BDNF level in CA3 area of Hippocampus. 4. The pre-interventions of medicine and non-medicine were both effective to helprats keep their learning and memory abilities. The combination with VC+VE andIbuprofen in medicine, low frequency rTMS in non-medicine were moreeffective to prevent rats from AD.Conclusion: The AD rat models established by co-injecting A P and Ibo into bilateral nucleus basalis of Meynet can simulate AD's severe learning and memory impairments. This kind of AD model is more suitable for the study of AD prevention. rTMS, NSAIDs and AO are all effective to prevent rats from AD. Furthermore, low frequency rTMS has more effects than high frequency rTMS does on the neurons, synapses as well as learning and memory abilities caused by co-injecting A P and Ibo for AD model's being established. In medicine intervention, the combination with NSAIDs and AO is recommended in AD prevention.