| Background: From twenty years ago, people began exploring the treatment and rehabilitation of ischemic cerebral vascular disease. Transcranial Magnetic Stimulation (Transcranial Magnetic stimulation, TMS) was found in 1985 for the first time, which uses the principle of electromagnetic induction. It was induced electromagnetic induction in the cerebral cortex using time-varying magnetic field, which streams along the pyramidal tract to the target tissue, then recorded the MEP (motor evoked potential, MEP) in the target tissues. As a non-invasive stimulation, it is easy to be accepted by the subjects. We can collect some important electro-physiology information of the nervous system by using TMS technology, such as MEP, motion threshold (motor threshold, MT), which can help us to predict cranial vascular disease. Depending on the development of TMS technology, Repetitive Transcranial Magnetic Stimulation (rTMS) was found in the 1990s. Different from the single pulse using in TMS, it can launch the stimulation pulse in a certain intensity and frequency within a certain time interval. A large number of experimental studies found TMS can significantly affect the metabolism, cortical function and cerebral blood in the brain tissue and associated area, change the balance between excitatory and inhibitory exited in the cortex, which can result in a series of secondary effects. RTMS attract many scholars from different academic fields because of its speciality, including public medicine, neuroscience and psychiatry. Scholars have founded that different stimulating parameters arouse the different result. Low-frequency rTMS can significantly reduced the level of regional cerebral blood flow and metabolism, on the contrary, high-frequency rTMS played an increasing role. As people begin to realize rTMS in a certain extent can improved the function of severe depression and movement disorder diseases, TMS making has become a common method of accessory treatment to cure depressive patients in some countries. But people know little about the effect of rTMS to Ischemic Cerebrovascular Disease. At this stage the experiments on animal and human has confirmed magnetic stimulation can change brain blood flow. Some other scholars prove that the rTMS on focal ischemia cerebral model rats can reduces brain infarction area and improve neural function. Pathological process of infarction mostly leads to the changes of Hemorheology. But there was no person to study the effect of rTMS to ischemia disease from the side of Hemorheology. In this paper we want to explore the effect of rTMS to the acute period of cerebral ischemia model in rat, discuss the possibilities of rTMS can be used in the recover process of cerebral ischemia disease.Methods: Create the model of cerebral ischemia in rats using the method of two vascular occlusion and keeping low voltage, we separately give rTMS to the rat in stimulation group 1h, 3h, 5h, 9H, 11H, 18h after the model making, every rTMS includes 10 sequence with each sequence last 5s and keeping a 2min spacing distance each other. Select high frequency and intensity stimulation with the parameters of 20Hz, 200%MT. collect 2.5ml blood from the rat tail 20h after the model making, measure the viscosity in three different shears and erythrocyte sedimentation rate(ESR) with Hemorrheology instrument. Compare the difference of Hemorrheology parameter among the different group rats. Analysis the effects of rTMS to these Hemorrheology parameter.Results: We found the parameters of rats in cerebral ischemia treated group obviously higher than in sham-operated group, including blood viscosity and ESR. It prompts that the erythrocyte aggregation has rised. After the rTMS treated the parameter of high cut and low cut whole blood viscosity obviously reduce compare with cerebral ischemia treated rats. We come to a conclusion that erythrocyte aggregation in rTMS group has reduced, leading to the improvement of whole blood viscosity.Conclusion: The result in this paper shows repetitive Transcranial Magnetic Stimulation (rTMS) can improve blood viscosity and erythrocyte aggregation after transient focal cerebral ischemia. rTMS can improve blood circulation, protect the cerebral ischemic from the damage. We predict the rTMS has a broad prospect in cerebral ischemia treatment. As a preliminary study, the methods using in this paper also need to improve in future. Because of the limitation of rat sample, effect of rTMS to cerebral ischemia disease is still needed to prove in further time. Innovation: In this paper we firstly observe the influence of Transcranial Magnetic Stimulation (rTMS) on Hemorrheology parameter of cerebral ischemia model in rat.
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