The Study Of Modulating Mechanism About Microvascular Endothelilal Cell Of Curing Ischemic Brain Injury From Removing Toxin To Dredge Brain Collaterals

Stroke is becoming a leading captured item in the domain of medicine because of its high incidence, high mortality, high deformity-causing. There is a history of several thousands years in understanding and curing stroke. Recently, delightful achievements have been acquired from the aspects of clinic and experiment, but a lot of problem is still desiderated. There should be a durance in bringing into predominant play in Chinese medical clinic and international spread due to short of arguable and repetitious quality in pathogenesis of stroke and ambiguous functionary mechanism of Chinese medicine. So it is imminence need to demonstrate pathological mechanism of stroke which is cured with Chinese medicine in modern science way. It is a Chinese medicine modernized way to dredge effect Chinese medicine from traditional medicine and prove its substance basement in experiment way. Academician Wang Yongyan put forward removing toxin to dredge brain collaterals that is meaningful in the combination of Morden Medicine and Traditional Chinese Medicine (TCM).In Chinese medicine, removing toxin to dredge brain collaterals as research key point is a break through to improve curative effect of cerebrovascular disease. It is realized that cerebral microvascular endothelial cells(CMEC) is not only a substance exchange barrier of blood and tissue, but also an important incretion apparatus. Our research pay more attention to CMEC rather than neurons, which is a meaningful probe to guide TCM realization mordernation used in brain disease.This study of drug is TongluoJiunao(TLJN) injection which is a traditional Chinese medicine employed in cerebral ischemia. The National Key Program of"pre-clinical research of TLJN injection"which has passed the test acquired outstanding effect in protecting rat injured tissue of brain ischemia. And its anti-ischemia damage effect has been further proved in clinical tests which was supported by"863"Research Projects. With study of CMEC as key target and NVU as a research system, we research pharmacologic mechanism of TLJN injection in cell, molecule and gene levers. The aim of this research is a meaningful supplement and an active probe of prevention and cure brain disease in theory and method aspects.Experiment is divided three parts:...