Comparative Proteomic Study Of The Pathogenesis Of Autosomal Dominant Polycystic Kidney Disease

We combined two dimensional liquid chromatography—tandem mass spectrometry (2D LC-MS/MS) with the method of isotope-coded affinity tag (ICAT) to do comparative analysis between the proteomes of urine, serum and cells from ADPKD patients and normal controls, aiming to find as many proteins as possible which get involved in the pathogenesis of Autosomal dominant polycystic kidney disease (ADPKD). Some of the identified differential proteins were chosen to be testified on their abundance difference by immunoblotting or immunofluorescence staining & laser confocal scanning. According to the data from ICAT—2D LC-MS/MS, numerous proteins besides polycystin-1 changed their abundance in urine, serum and cyst-lining epithelial cells of ADPKD patients. These proteins include growth factors, apoptosis regulators, transporters, extracellular matrix proteins, receptors, cell signaling proteins, enzymes, transcription factors and regulators, as well as others. They may take part in the development and progression of ADPKD by affecting the biological processes such as proliferation, apoptosis, and vesicular transport of cyst-lining epithelial cells, interaction of cyst-lining epithelial cells with extracellular matrix and other cells, fibrosis of cysts,defence against bacteria, development of kidneys, etc.