Comparative Analysis Of Brain And Coronary Microvascular Endothelial Cells And The Roles Of Decorin In Cerebral Ischemia/reperfusion

We investigated the gene and protein expression in rat primary cultured brain microvascular endothelial cells(BMEC) and coronary microvascular endothelial cells(CMEC) using cDNA array and proteomic approach, then the roles of differentially expressed protein decorin in rat ischemic/ reperfusion model were studied.The results showed that there were extensive differences in RNA and protein expression in BMEC versus CMEC. BMEC were more involved in anti-inflammation and adhesion inhibition or in anti-oxidant and extracellular matrix-related molecules, while CMEC exhibited high expression of genes involved in pro-inflammatory cytokine and oxidant molecules. Using the proteomic approach, we comparatively analyzed the proteome of the cultured BMEC and CMEC. Our data suggested that BMEC and CMEC were different in several aspects including cytokine and growth-related molecules, stress-related proteins, metabolic enzymes, signal transduction proteins and others. Moreover, decorin expression increased after cerebral ischemia/reperfusion. Decorin also suppressed LPS-induced MCP-1 production by endothelial cells. The upregulation of decorin in rat ischemia-reperfusion models indicated that this proteoglycan may play important roles in repair processes after cerebral ischemia.