The Effects Of Aerobic Exercise And Different Fatty Acids Diet On Dyslipidemic-diabetic Hamster Model

Objective: Metabolic syndrome is a multiple genes, multiple factors disease, food construct and exercise appear to play important roles among environment in its development. The objective of this study was to investigate the effects of aerobic exercise and different n-3 fatty acids diet on high-fat induced dyslipidemic-diabetic hamsters model.Methods: 40 healthy male depuratory hamsters were divided into 4 groups: control group (10% lard), aerobic exercise (EX) group (10% lard), high α -linolenic acid (HALA) group (n6/n3,12.4:1) and low α -linolenic acid (LALA) group (n6/n3, 18.4:1). EX animals were treadmill trained 5 days per week. Animals' body weights were scaled per week. After 10 weeks experiment, the fasted 12h hamsters were anaesthetized by pentobarbital sodium, once the blood had been collected from arteria femoralis, hamsters were cervical dislocated and sacrificed. The animals were dissected, the internal organs, skeletal muscles, amphi-organs adipose tissue were excided off immediately, calculated these adipose tissues over body weights. Serum lipids, insulin, glucose, free fatty acids (FFA), triacylglycerol (TG) and FFA in insulin target tissues, and liver lipoprotion lipase (LPL), hepatic lipase (HP), apoprotein A-1 (ApoA1) and B(ApoB) were determined. Leptin in adipose tissue was assayed by enzyme immunoassay and tumor necrosis factor α (TNF- α ) in adipose tissue was measured by double anti-sandwich ELISA, peroxisome proliferator-activated receptor (PPARs) in liver and glucose transporter-4 (GluT-4) in skeletal muscles and adipose tissues were estimated by RT-PCR.Resu Its: The results showed that the body weights gain unchanged among groups. The fats of amphi-didymus over body weights of EX group was markedly smaller than others groups (P<0.01). The fats of amphi-muscles overbody weights were reduced than control group. EX and n-3 fatty acids decreased serum glucose and insulin (P<0.05 and P<0.01, respectively). But EX and enriched n-3 fatty acids diet effects on serum lipids, TG and FFA in liver, muscle, adipose tissue had not significantly changed. ApoAl in liver of EX group enhanced compared with control group (P<0.05), and LALA group had more markedly (P<0.01). Leptin in adipose tissue of EX group were up-regulation (P<0.05). EX and diet containing n-3 fatty acids did not decrease TNF- a in adipose tissue. PPARs in liver of HALA group hamsters markedly up-regulated, and PPAR Y had been more heightened. EX up-regulated GluT-4 in muscle and adipose tissue, compared with control group (P<0.01).Conclusion: Aerobic exercise and n-3 fatty acids could be diminution body fat masses, improvement insulin resistance (IR) by high-fat diet induced, decreased serum glucose and insulin. Aerobic exercise could increased leptin in liver and GluT-4 mRNA up-regulated in skeletal muscle and adipose tissues, improved insulin action on glucose utilization, decreased insulin resistance in insulin target tissues. Added n-3 fatty acids diet could regulate transcription factor activity and abundance, PPAR a and PPAR Y in liver gene expression up-regulated, restricted TG synthesis and aggregation, improved fatty acids burning, enhanced insulin sensitivity. Regularity exercise and dietary n-3 fatty acids healthy diet appeared to play important protecting roles against the adverse symptoms of the "Plurimetabolic syndrome", preventing or ameliorating coronary heart disease and strike.