Expression And Function Of TGF-β/Smads Signaling Pathway In Renal Interstitial Fibrosis And Effects Of Simvastatin And Valsartan On Its

Objectives1. Renal interstitial fibrosis is the common pathological procedure to renal function failure of many kinds of renal diseases. Its pathogenic mechanism is not clarified to this day in which many factors is involved. Transforming growth factor-βis one of the most important factors in renal fibrosis. It plays key role in the progression of renal interstitial fibrosis as. Smads protein family is a new cluster of proteins which mediates intracellular signal transduction of TGF-β. They translocate signals from the cell surface to the nucleus where they regulate TGF-βsuperfamily-dependent gene expression. In this study , SD rats were used to induce renal interstitial fibrosis model by unilateral ureteral obstruction and human kidney proximal tubular epithelia cells were cultured and stimulated by recombinant TGF-β1 to investigate the relations of TGF-?/Smads signaling pathway to extracellular matrix accumulation , cell apoptosis and proliferation.2. Statins is well known by its therapeutical effect on high blood lipid. Abundant experimental and clinical investigations have proved its renal protective effect independent of lipid reduction function. Rennin angiotensin system, especially Ang II, is important in renal glomerulosclerosis and interstitial fibrosis. Valsartan is a kind of angiotensis receptor blockade which not only ameliorate hypertension but also protect target organ uniquely. Simvastatin and Valsartan were chosen to investigate their effects on TGF-?/Smads signaling pathway, further to clarify renal protection mechanism of them.Methods1. Experimental model and detection of TGF- ? /Smads signaling...