Experiment Study On Ultrasound Combined With Contrast Agent Enhancement Transfection Of VEGF Gene In Vitro And Vivo

Gene therapy has the potential to become the ultimate conquest of many disobedient diseases, and is also one of the most important realms in medical molecule-biology for the time being. However, there are a lot of obstacles impede gene therapy's being extensively applied clinically. How to make target gene into specific organ,tissue of human body securely,efficientively,orientedly and express in target cells is still one of the formidable problems to be tackled.Currently, the methods of gene transfection in vivo all have specific limitation. Gene transfection taking virus as vector has a high efficiency, but its safety always be the concern of people, the immune reaction of the body to virus is also a problem that difficult to surmount at the same time. The method of the non-virus gene transfection taking lipofectine as representative turn away the shortcomings above-mentioned but have a lower efficiency in vivo than cultured cells in vitro. Besides the cytotoxicity, lipofectine is susceptible to the disturbance of fat,lipoprotein and extracellular matrix, which limited its application in vivo. While the nonviral vector, plasmid can evade the immune reaction of caused by viral vector and cytotoxicity evoked by lipofectine. The target gene will not be integrated into host cells. So the safety of plasmid is much more reliable. So link the target gene to expressed plasmid and inject them...