Studies Of Metastasis-related Mechanisms Of Gastrointestinal Cancer Based On TMA And Gene-chip

Objective: To compare the difference of gene profiles between primary tumor cells and metastatic tumor cells of gastrointestinal carcinoma and search for new genes or new functions of genes. Methods: Tissue microarrays of 112 gastrointestinal cancers were constructed. Immunohistochemistry, LOH, Gene chip, Real-time RT-PCR were used. Certain methods were taken to inhibit the pathway of Grb2 and to observe its role in invasion. Results: IHC revealed higher expression of nm23 and KAI1 in primary tumor than in metastatic tumor. The rates of nm23, p53, and DCC LOH increased during the process to metastasis of cancer cells. Sixty-three genes were up-regulated and 160 genes were down-regulated in metastatic cells than in primary cancer cells. Grb2 was more common and extremely higher in metastatic tissues than primary tumor tissues. Then we used Grb2-SH2 domain-binding antagonist to counterpart its role of metastasis potential in Colorectal cancer cell lines, which turned to be effective. Conclusion: Multiple genes contribute to the progression of metastasis of Colorectal carcinoma, which may be the target of treating this disease. Metastasis-related genes could be found by...