Human Adipose Tissue Derived Stem Cells Transfected By EGF Gene Differentiate Into Epidermal Cell In Vitro For Wound Healing

Skin defects after tumor or scar resection, burns injury, other injury or chronic ulcer after system disease such as diabetes or vascular diseases are the main problems in plastics and burns surgery. For the superficial degree burns or small area skin defect, the wound can heal through the regeneration of the local remained stem cells or the epidermal cells which are from the adjacent tissue to immigrant to the wound site. for the deeper degree burns such as III degree burns or a large area skin defect the wound have to be covered with autograft or skin substitute by tissue engineering. These treatments have their disadvantages, which include resulting in a severe morbidity of donor site, no enough autografts provide with large area burns, and the allografts maybe result in a immune exclusive reaction. At present the tissue engineering skin, which has been applied in the clinical practice, still result in variable degree of immune exclusive reaction and the clinical result is not very good. The formation of scar have a close relationship with the time of wound healing, the more length the procedure of wound healing, the more collagen of wound deposit and the more severe scar form, so to find out the way of improving the wound healing is very important strategy in clinical work.Recently the developing technique of stem cells provides a new strategy and method for the wound healing. Stem cells are comprised of embryonic stem cells (ESCs) and adult stem cells. The practical use of ESCs is limited due to potential problems of cell regulation and ethical...