| ObjectiveEpidermal growth factor for the positive role of wound healing, rhEGF adjustable in many areas of the skin wound healing. In this study, through the establishment of co-burn rat model of diabetes, wound healing, local injection of insulin to control blood sugar based on the combination with topical rhEGF, detection rate of wound healing, bFGF, CK19 protein, of rhEGF promote wound healing potential of diabetic Diabetic wound healing mechanisms and the possible mechanism.Methods160 Wistar rats were randomly divided into A, B, C, D 4 groups。Rats of group A, B, C were injected with streptozotocin to induce diabetic models. Diabetes models were confirmed by testing blood glucose and pathology and immunohistochemistry verified destruction of pancreatic islets and insulin secretion. Group D as normal control group (D) also were produced deepⅡdegree burn model. Each group were used different interventions: group A : Combination of insulin and rhEGF group (Wound and insulin dose of insulin and other local infiltration, and spraying with rhEGF group outside the same dose of rhEGF); group B: Spraying the rhEGF group, wound Spraying the amount of rhEGF; group C: insulin, local infiltration of the wound were injected with insulin; groupD: normal control group, the wound was dealed with saline.,,and the wounds were bandaged medication. The rate of wound healing and the expression of growth factors bFGF,keratin K19.in the wound healing process at different time points were detected.Results1. Group B and group C was no significant difference in healing rate at each time points (P> 0.05), group A is higher than the B, C groups in each points (P <0.05), group A and group D has no significant difference in healing rates (P> 0.05).2.The bFGF protein of group A, D at different time points are higher than the group B, C; and there are no differences between the groups D, Aas same as between the groups B, C at each time points (P <0.05).3. CK19 mainly distributes by the increase to double and even multi-layer, and epithelial cell proliferationobserved in the epithelial islands gathered. Group D with higher levels of CK19 protein, is higher than groups B,C (P <0.05);Groups B,C have no significant difference as well as groups A,D .Conclusion1.The external application of rhEGF and controlling blood sugar, can obviously promotes wound healing of diabetes burn patients. 2.Thorough debridement and dressing for rhEGF can increase the contact time, local infiltration injection of long-acting insulin ,which may also be beneficial to thesustained effect, the synergy between the two providing a good fix for the woundmicroenvironment. 3.United rhEGF external glucose control in diabetic wound healing process, can promote wound fibroblast growth factor expression of bFGF to promote wound healing.4. In the base of controlling blood sugar, on the continuous stimulation of exogenous EGF can significantly stimulate the wound ck19 expression, and promote proliferation and differentiation of ESCS is conducive to wound re-epithelialization.5.Some diabetic wounds are too low in the expression of cell factors and its receptors or inhibition .The wound proliferation and differentiation of epidermal stem cells is caused by diabetes and other factors are the important reasons of the delayed wound healing .
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