Study On The Mass Spectrometric Analytical Method Of Flavonoid Glycosides

A series of flavonoid glycosides including flavonoid O-glycosides, flavonoid O-diglycosides, flavonol O-acetylglycosides and flavonol 3,7-di-Oglycosides were systematically investigated by positive and negative ion electrospray ionization tandem mass spectrometry (ESI-MS~n ). The relationship between their structural features and the corresponding characteristic fragmentation behavior was investigated and the formation of characteristic ions and the factors influencing theirs intensities were also discussed. Particularly, a novel analytical idea was put forwarded to determine the glycosylation position and saccharidic sequence. At last, rapid and effective analytical method were established through investigating and summarizing the fragmentation behavior of these compounds which can be benefit to analyze the related constituents in medicinal plant crude extracts. Based on the fragmentation rules and analytical methods established previously, three crude extracts were analyzed by ESI-MS~n, LC-UV-MS and LC-UV-MS~n techniques, and the possible structures of thirty-one components including known and unknown compounds were inferred, some of them were confirmed by comparing with the standards.1. Isolation and identification of flavonoid compounds from Salix caprea L.Flavonoid constituents in the pollen of Salix caprea Z.(Uyghur Medicinal Plant) were isolated by chromatographic techniques, and eleven main constituents were obtained. Their structures were identified by NMR and MS techniques. The structure of these compounds are as follows: isorhamnetin 3-O-β-D-(6"-O-acetyl)- glucoside(l), kaemferol 3-O-β-D-(6"-O-acetyl)-glucoside(2), quercetin 3-O-β-D-(6"-O-acety)-glucoside(3), quercetin 3-O-β-D-glucoside(4), isorhamnetin 3-O-β-D-glucoside glucoside(5), myricetin 3-O-β-D-glucoside(6), kaempferol 3-O-rutinoside(7), quercetin 3-O-rutinoside(8), quercetin 3,7-di-O-glucoside(9), isorhamnetin 3,7-di-Oglucoside(10), myricetin 3,7-di-Oglucoside(11). Except compound 10, all compounds were isolated from pollen of Salix caprea L for the first time. This study...