Proteomic Study Of Protein Kinase Cε Signaling Complexes In Rat Myocardium During The Development Of Hypertrophy, Reversing Hypertrophy And Heart Failure

Objective:To discuss the establishment and criterior of diagnosis of pressure-overload myocardial hypertrophy (POH), hypertrophy-reverse (POH-Re) and chronic congestive heart failure (HF) rat models. Methods:8-week-old Sprague-Dawley male rats were randomly divided into two groups: abdominal aortic constriction group (AAC) and sham group. AAC rats were performed abdominal aortic constriction to form POH, and age-matched rats were performed sham to serve as controls. After 12 weeks of surgery, all rats were performed echocardiography, and AAC rats whose LVmass/BW from echocardiographic detection increased were divided randomly into myocardial hypertrophy group (POH-NF, n=20) which were killed immediately, hypertrophy-reverse group (POH-Re, n=12) treated with Carvedilol (Roche, 0.5mg/kg/day) and Fosinopril (Squibb, 10mg/kg/day) for 8 weeks before being killed, and heart failure group (POH-F, n=29) on high-salt diet for 12 weeks before being killed. All the rats were detected their echocardiographic data and hemodynamic parameters through cadiocatheter via carotid artery before being killed. Wet weights of heart and lung were weighed, moreover, a part of heart and lung was prepared for morphological observations, and another part of heart was flash-frozen for biochemistry analysis. Results:...