Research Of The Effect And Mechanism Of Surgical Delay On The Survival Of Neuro-island Flap Based On The Intrinsic Vasculature Of Its Cutaneous Nerve

Objective: To Study whether an acutely elevated skin flap can survive based solely on the intrinsic vasculature of its cutaneous nerve and the effect of the surgical delay on the survival of this kind of flap and the changes of microcirculation of delayed skin flaps and explor the effects of nitric oxide(NO) and vascular endothelium growth factor (VEGF) and basic fibroblast growth factor (bFGF) on surgical delay.Methods:The modol of inferior epigastric neuro-island flap based on the intrinsic vasculature of its cutaneous nerve was designed in the rat and the survival of the delayed group was compared with that of the nondelayed group. The changes of microcirculation of delayed skin flaps was observed through HE stain and qualitative and quantitative analysis of VEGF and bFGF was completed by immunohistochemical technique and ELISA. After delay procedure, each animal received an intraperitoneal dose of L-NAME, a nitric oxide synthase inhibitor, at a level of 0, 50, 100mg/kg/day and the survival of each group was compared. The NO metabolic product was messured through Griess method.Results: The survived area of the acutely elevated inferior epigastric neuro-island flap based on the intrinsic vasculature of its cutaneous nerve in rat was very small. The average survival percentage of the delayed group was higher than that of nondelayed group (p<0. 05) . Vasodilatation and neovascularization was observed from the tissue biopsied 3 days after surgical procedure. The contents of NO metabolic product and the expressions of VEGF and bFGF are increased significantly. The difference in the mean percentage survival area between each groups...