| A molecular epidemiology investigation of human papillomavirus(HPV) on condyloma acuminatum and analysis of intratype nucleotide and amino sequence variation of HPV6 L1 ORF were performed, which would help to trace the pathways of transmission of HPV infections and assist in establishing genotype and phenotype relationships as well as to aid in the development of vaccine strains.To identify and assess multiple human papillomavirus types in condyloma acuminatum lesions from patients in Beijing area, universal primer MY09/MY11 was used to amplify conserved sequence in HPV late gene L1 region. Polymerase chain reaction (PCR) and restriction fragment length polymorphism (RFLP) were performed to determine HPV types from individual lesions. For specific type which could not be determined by RFLP, recombinant sequencing plasmid was constructed for further identification. conclusion was assessed according to comparison with Genbank. Results revealed that the predominant type from otherwise healthy patients was HPV6, secondly HPV11. The mean age of patients infected by HPV6 was lower than that of HPV11 and HPV6+11. While lesions from immunosuppressed patients most often contained HPV11 or mixed with HPV6. Besides, HPV16 as a mixed-infection pathogen could be detected from some female patients. As a low-risk virus in normal genital tract, HPV53 also could be a pathogen in genital warts.For predominant type, eight isolates of HPV6 with different clinical manifestation were chosen for further intratype nucleotide and amino sequence variation analysis. HPV6 L1 genes were amplified and inserted into plasmid pMD18-T. By sequencing and phylogenetic analysis, the most frequently observed substitutions in HPV6 L1 ORF were distributed into four discrete regions: SR1(5911~6104), SR2(6217~6273), SR3(6540~6661)and SR4(7062~7250).
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