Study On The Mechanisms Of Burns Repair

With the development of molecular, cell and developmental biology, we have made great progress in understanding the biologic process involved in wound repair and tissue regeneration. The wound repair is a very complex process requiring the collaborative efforts of many kind of tissues and cell lineages. Perfect skin regeneration not only involves perfect regeneration of epidermis and dermis, but also involves the repair of skin appendages, vasculature and peripheral nerve. Moist Expose Burn Ointment (MEBO) treatment is a novel technique to treat wound. The clinical experiments showed thatMEBO could promote wound healing and reduce the incidence of scarring and infection. Although MEBO had been used clinicly for many years, the mechanisms of promotion of wound healing are unclear. The purpose of this paper is to investigate the spatio-temporal profile of typeI collagen, MMP-1, integrinα₃, integrinβ₁, TGF-β₁ and Smad3 during wound repair. Meanwhile, The relationship of MMP-1, integrinα3, integrinβ1, and Smad3 was investigated, Their functions were also explored. The theory and experimental basis for investigating the functions of these molecules (especially Smad3) during wound healing were provided.First, pathological changes during wound healing after using MEBO were observed. After wound 24hours, there was no fibrin clot on the wound site and the inflammation reaction was slight. On day 4, Coagulated sphacelus were bigger, inflammation cells infiltrated, edema in wound site was found. On day 7, there was thick exudate on the surface of wound site. Coagulated sphacelus lied down beneath the exudate, inflammation cells surrounded the coagulated sphacelus. In this phase, The inflammation reaction was...