The Evaluation Of Gene Gun-mediated DNA Vaccine PWRG-neu Immunization Inhibited Neu-overexpressed Tumor Growth And Metastasis

DNA immunization has been shown to elicit both strong and persistent antibody and CTL responses against antigens synthesized in vivo after direct introduction of the DNA encoding sequences and therefore is potent to induce antitumor effects. In this study, we evaluated the antitumor effects of gene gun-mediated c-erbB-2 DNA vaccine pWRG-neu in C57BL/6 mice.At the beginning of this study, we tested the transfection efficiency of Helios gene gun. With Helios gene gun, two kinds of reported genes Lac-Z and EGFP were transfected to several cultured mammalian tumor cell lines in vitro and mice skin in vivo, respectively. A stable long time exogene's expression was observed. Then, the pWRG3142, which carried mGM-CSF gene, was delivered to the abdominal skin of C57BL/6 mice using Helios gene gun. Pathological sections showed the local transproteins' expression accompanying by a profound inflammation reaction characterized by neutrophilic infiltration. ELISA assay of transgenic mouse's serum sample indicated a high improvement of transgenic protein level, which demonstrated that transgenic GM-CSF secreted from treated skin into the bloodstream effectively. In B16F10 melanoma tumor model, mice immunized with mGM-CSF expression plasmids could be partially protected from 1×10~5 B16F10 cells challenge and exhibited a drastically reduced tumor growth rate. From these study we resulted that exogenes could be transfected into target effectively in vitro and in vivo by gene gun technology, and gene gun-mediated in...