Apoptosis Of Human Pancreatic Carcinoma Cells Induced By Chemotherapeutic Drugs And Its Mechanism

Pancreatic carcinoma is an aggressive disease and the patients typically have a short survival. Despite recent improvements in diagnosis and staging, the prognosis remain poor with low 5-yr survival rate of 5%. The overall fatal outcome of this disease is because of frequently the tumor at the time of diagnoses has either already metastasized or is locally advanced , so that in most case resection without tumor rests is not possible. The median survival of patients without surgical resection is only 3-6 months.There is, therefore, a need to develop selective and effective adjuvant treatment directed at reducing the morbidity and mortality associated with pancreatic carcinoma.Today, the chemotherapy is one of the most important adjuvant treatment for pancreatic carcinoma. For the patients, the chemotherapy can delay their survival time, improve the quahty of their lives and reduce their pain. Therefore, there is important to study the sensitivities to chemotherapeutic drugs in human pancreatic carcinoma cells and its mechanism in vitro, which can help us to make new and best chemotherapeutic regimen and give some good ideas for our clinical work.MTT is a semi-automated colorometric assay which is currently being successfully applied to the chemosensitivity testing of chemotherapeutic agents on tumor cell lines in the world. In the study, the chemosensitivity of 5-FU, MMC, CBP, ADM, E-ADM and their combinations on human pancreatic carcinoma cells were tested. The cells of pancreatic carcinoma cell lines (P3, SW1990, Capan-2)...