1.Radioimmunoassay For Osteocalcin And Its Clinical Significance 2.17 Beta-estradial Influence Calbindin D9K Gene Expression In The Ovariectomized Rat Intestines

We extracted and purified osteocalcin(BGP) from calf femur by gel filtration, FPLC and HPLC subsequently. The rabbits were immunized by this antigen and produced antibody. With this antibody(final 1:35000 dilution) we established RIA for BGP. This assay is stable and sensitive, the Kd is 2.29 10. The inter assay variation was 2.1% and the intraassay variaton was 5.6%. It has no crossreaction with insulin, parathyroid hormone or calcitonin.The serum BGP values were detected in normal subjects and six bone metabolic diseases. In 81 normal subjects, the average serum BGP level is 5.36±1.56 ng/ml, in postmenoposal osteoporosis, the serum BGP level (6.12±1.97ng/ml) is higher than the normal (p<0.01). The serum BGP levels were increased much in primary hyperparathyroidism (12.78±7.03ng/ml, n=21, p<0.001). Increased values of BGP were also found in patients with chronic renal failure (8.532±2.45ng/ml, n=12, p<0.001) and Paget's disease (6.65±2.21ng/ml, n=12, p<0.01). Decreased serum BGP values were found in Cushing syndrome (2.89±0.92ng/ml, n=16, p<0.001) and hypoparathyroidism (3.37±1.18ng/ml, n=42, p<0.001). This result shows that in diseases with high bone turnover, the serum BGP levels are increased , in diseases with low bone turnover, the serum BGP levels are decreased. The data suggests that serum BGP is a sensitive marker for bone turnover. It is very important in studying bone metabolic disease.