| One of the characteristics of malignant cells is deviation from the normal differentiation pattern toward a more undi-fferentiated state. Whatever redirects such cells toward differentiation is likely to decrease their malignant potential, since fully differentiated cells are more subject to the mechanism in harmony with other cells in the body. In this study, we investigated the modulation of monoclonal antibodies (MoAbs) to human pancreatic carcinoma on the PANC-1 cell growth and differentiation.First of all, we generated a panel of murine MoAbs to human pancreatic carcinima, and further characterized six of these MoAbs. The reactivity of the six MoAbs with human tumor cell lines in culture was studied with RIA. As a general rule, the antibodies reacted with some tumor cell lines of epithelial derivation, and no significant reactivity was observed with leukemic and lymphoid cell lines, human RBC and lymphocytes. In order to further confirm the results, we also performed cell-ELISA. To attach cells to plates, the widely used poly-L-lysin was not suitable in our laboratory. So we tried to find a new substance which could replace poly-Llysin. The improved method had several advantages: cheep, easy to get and high degree of reproducibility. The results of the two assays were resemblance.The tissue distribution of the antigens recognized by the six MoAbs was studied by immunoflurescence staining. Of the six antibodies, only PM1, PM2, PM4 and PM3 reacted with each pancreatic carcinoma specimen tested. The remaining antibodies were negative with one pancreatic carcinoma specimen. At the same time, we tested twenty-eight other human tumour specimens, and each MoAb with different reactivity was observed. Although the panel of human tumour tissues tested was somewhat limited due to the scarcity of such specimens, the results supported the possibility of considerable antigenic heterogeneity of the tumour-associatd antigens. In order to determine the degree of tumour specificity of these MoAbs.
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