Biological Characters Of Human Eccrine Sweat Gland Epithelial Cells And Three-dimensional Reconstruction Of Eccrine Sweat Glands In Vitro

The human eccrine sweat gland is an important skin accessory involved in secretion, excretion and thermoregulation . To separate and culture eccrine sweat gland epithelial cells in vitro would be of value in the study of its structures and functions further, in the understanding of fluid secretory mechanisms and in the elucidation of epithelial ion transport procedures and the mechanism of tubulogenesis. Furthermore, culturing the eccrine sweat gland cells in vitro makes it possible to fabricate the tissue engineering skin with accessories. At present, there are a few reports on sweat gland epithelial cells cultivation , which are only limited to tissue explant cultures and primary cultures. The cultured cells were impure and it took a long time to reach confluency. It is necessary to develop a more effective culture technique for isolating and culturing human eccrine sweat gland epithelial cells and try to reconstruct sweat glands in vitro.Therefore, this study was to establish a new and effective method to culture human eccrine sweat gland epithelial cells, to investigate the biological characters of the cells and possibility to reconstruct the sweat glands in vitro. Cell culture, immunohistochemistry, histopathology, westernblot and CLSM were used in this study. The methods, results and conclusions are shown as follows:Methods: 1. Human eccrine sweat gland epithelial cells were separated by typeâ…¡collagenase digestion and cultured in KSFM, the harvested cells were identified by immunohistochemistry and electron microscope, and the biological characters of cells were observed.2. The effects of acetylcholine(ACh) and ATP on [Ca2+]i of culturing sweat gland epithelial cells were studied by CLSM.3. The effect of HGF on proliferation of eccrine sweat gland epithelial cells was investigated by MTT, the effects of HGF on migration and morophogenesis of cells were...