Expression And Localization Of AQP5in Axillary Sweat Glands Of Palmar Hyperhidrosis With Axillary Hyperhidrosis Patients And Experimental Study Of Sweat Gland Epithelium In Vitro Model

Objective:The aim of this study was to explore the relationship between AQP5and palmarhyperhidrosis through detected the expression and localization of AQP5in patients’axillary sweat glands and research in vitro model of the human sweat gland cells.Methods:The axillary skin and serum specimens of patients with severe palmarhyperhidrosis with axillary hyperhidrosis (Experimental group) and non-hyperhidrosispatients (Control group) were collected. The morphological differences between thetwo groups were observed by using HE staining and transmission electron microscopy,count the sweat gland cell secretory granules via electron microscopy, and detectAQP5expression by using immunohistochemistry, quantitative PCR andwestern-blot.Sweat gland epithelium in two groups were trained by using enzyme digestion. Thecells were identified by using immunocytochemistry. The cell growthtrend and AQP5expression were compared between two groups. The serum concentration ofacetylcholine was detected by using Elisa, and formulated culture medium for cellculture which configured in accordance with the measured concentration. Theexpression of AQP5in sweat gland cells was detected by using immunofluorescenceand western-blot.Results:It was no significant difference between two groups in the pathological morphologyand ultrastructure. Secretory granules and expression of AQP5in cells of theexperimental group were higher than that in the control group. The cells expressed CK7, CK19, but unexpressed actin, and the cell growth trend in two groups wassimilar. The level of AQP5mRNA in experimental group was higher than that in thecontrol group (P <0.05), while the protein was no significant difference (P>0.05).The peripheral acetylcholine concentration in experimental group was higher thanthat in the control group（P<0.05）. The expression of APQ5in sweat gland cellscultured in high concentration medium was significantly higher than the low-dosegroup(P <0.05) after6h.Conclusions:There were no difference in the pathological morphology of sweat gland betweentwo groups. The expression of AQP5was up-regulated, and the level of theacetylcholine was elevated in experimental group. It indicated that the excess ofneurotransmitter (acetylcholine) release and the up-regulation of AQP5may beassociated with the hyperhidrosis pathogenesis.In vitro culture, the expression level of AQP5mRNA was higher than that in controlgroup, but the expression of the AQP5protein was no significant difference. With thestimulation of the Ach, the expression of AQP5in two groups were elevated, and risedwith the concentration. It prompted that the up-expression of AQP5in patients withhyperhidrosis may be related to the neurohumoral regulation.