The Experimental Study On The Construction Of Skin Sheet With Two Kinds Of Gene Modified Cells And Its Employment In Promoting The Healing Of Wound-Radiation Combined Injury

The wound combined with radiation injury of full-thickness skin often occurs in patients with radiotherapy after surgical operation and in patients suffering from radiation injury combined with explosive injury resulted from nuclear accident. The wound combined with radiation injury of full-thickness skin can also occur in nuclear detonation and the using of depleted uranium weapon during wartime. The local tissue wound is usually accompanied with whole body injury. The two aspects deteriorate each other and increase great difficulty to the treatment.At present, the approaches to promote the wound healing of full-thickness skin tissue defect include the application of chemical medicine, new biological and traditional dressings, skin graft, cultured cell layer, cytokines, and gene therapy. However, the application of chemical medicine, new biological and traditional dressings only show efficiency in certain extent; Skin graft is the most effective treatment in wide soft tissue deficiency, but the resource of skin for autograft or allograft is limited; Skin allograft and artificial skin equivalent cannot break through the critical issue of immune rejection; Cultured cell layer is thin and fragile; Studies have confirmed the single or combined application of exogenous cytokines at wound site can accelerate wound tissue repair. Due to the half life of growth factor is only several hours or even shorter, and the growth factor wouldn't take into effect if there is not enough time for them contact with cells at wound site. As polypeptide and protein, growth factor can be limited due to their inactivation by protease presented in wounds.In recent years, the molecular genetic approach is becoming a novel measure for treating tissue repair and wound healing. By employing gene transfer technique, exogenous genes with specific function can be delivered into wound site, and the desired growth factor protein is stable expressed, released during the process of tissue repair, aiming at accelerating and...