| Mitotic arrest deficient 2 (MAD2) is an essential component of the mitotic spindle checkpoint pathway and plays a vital role in maintaining spindle checkpoint function by generating "waiting anaphase" signal.During cell cycle progression, there are three checkpoints that monitor a series of events to ensure faithful replication of the genome and accurate alignment of chromosome prior to cell division. One of them, spindle checkpoint, is thought to be indispensable for chromosome stability. MAD2 was found to be differentially expressed between SGC7901 and SGC7901/ADR cells by streptavidin magnetic beads mediated subtraction cloning method in our previous research. And further study found a fragment of 0.53 Kb, which was confirmed as a new alternative splicing form of MAD2 (MAD2β) by DNA sequencing, and the splicer could increase the multidrug resistance of SGC7901 cell line. These studies indicated that the status of MAD2β might be ery important for determining a resistance towards chemodrugs.[Purpose] The purposes of present study are as following: 1. To further validate the effects of MAD2β on MDR of gastric cancer cells and explore the mechanisms; 2. To study the roles of spindle checkpoint in maintaining the sensitivities of gastric cancer cells to chemodrugs; 3. To explore the mechanisms of by which MAD2 regulated MDR of gastric cancer cells.
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