Protective Effects And Mechanisms Of Hyperoxygenated Solution On Intestine And Remote Organs Injuries Induced By Intestinal Ischemia Reperfusion

AIMS: Rabbit intestinal I/R model was established to observe intestine and distant organs injuries induced by intestinal ischemia reperfusion, and investigate the protective effects and potential mechanisms of intravenous infusion of hyper-oxygenated solution (HOS), HOS preconditioning, intraluminal HOS infusion and HOS infusion via SMA on these injuries.METHODS:1) Preparation of HOS: Medical oxygen is introduced into the "Medical hyper-oxygenated solution apparatus" at an inflow of 3L/min for 15min. Treated with ultraviolet light (wave length=180-240μm), some oxygen transforms into ozone. The oxygen/ozone mixture flows into the airtight base solution (500mlnormal saline), and this base solution turns into (HOS).2) Rabbit intestinal IR model: Male New Zealand white rabbits (2-3kg) wereused in this study. After an overnight fast with unrestricted access to water, the animals were anesthetized intravenously with 3% pentobarbital sodium (30mg/kg). After a midline laparotomy, the superior mesenteric artery was occluded for 60min with an atraumatic vascular clamp, followed by reperfusion for 2hours.