1. The Correlation Of The Differential Expression Of S100A4 Gene(mts1) With The Differentiation And Metastasis Of Lung Cancer 2. Effects Of HuTNF-α,hIL-2 Gene Transfection On The Expression Of MDR1,LRP Gene In Lung Cancer

Objective: Invasion and metastasis are hallmarks of lung cancer and other malignant tumors. The activation of many genes has been shown to be important in tumor progression. We studied the corelationship of S100A4 gene (mts 1) expression with the prognosis evaluation of lung cancer. Methods: Total RNAs were extracted from 46 cases of patient with lung cancer, and 4 lung cancer cell lines ( A549,GLC-82,NCI-H446, NCI-H460 ) , S100A4 gene expressions were detected at mRNA level by reverse transcription-polymerase chain reaction (RT-PCR). Results: Up-regulation of S100A4 gene was shown in 29 of 46 (63.0%) lung cancer patients and all of the lung cancer celi lines, especially in younger patients( 89.5% vs 44.4%, p <0.01 ), poor differentiated lung cancer patients( 81.0% vs 48.0%, p <0.05 ), mediastinum lymphonodus metastasis (77.3% vs 20%, p<0.05 )and the IIIa stage patients( 73.1% vs 0 ,p <0.05 ). Conclusions: S100A4 gene played an important role in lung cancer progression, correlated well with the condition of the lung cancer differentiation, mediastinum lymphonodus metastasis, and the prognosis for the patients of lung cancer. It was likely that S100A4 gene as a prognostic, predictive, or even diagnostic factor in clinical practice.