Function And Mechanism Studies On MTA1 Gene In Cancer Invasion And Metastasis

Cancer is the disease seriously threatening the health and survival of people. Till now, the detailed mechanisms on metastasis of esophageal squamous cell carcinoma are unclear. This study took the metastasis associated gene 1 (MTA1) as the target to make further understanding about metastasis. The MTA1 product is a component of nuclear remodeling and deacetylation complex (NuRD), which regulates the gene transcription by altering the chromosin status. It is reported that MTA1 is associated with the high metastasis potential in many types of cancer cells.We analyzed the pathological specimens on tissue microarry using immunohistochemistry. MTA1 expression increased in the nuclear with the development of esophageal squamous cell carcinoma from normal epithelial cell, dysplasia, invasive cancer to metastasis cancer.The study in human esophageal squamous cell carcinoma cell line KYSE150 and mouse melanoma cell line B16F10 supported the MTA1 function to promote invasion and migration. Boyden Chamber model was adopted to study the in vitro invasion and migration. The siRNA transfection against MTA1 decreased the ability of cancer cell to invade through 8um pore polycarbonate pre-coated with Matrigel ECM. When the polycarbonate is pre-coated with fibronectin, the number of migrating to the lower side of the membrane indicated the in vitro migration capacity of cancer cell. In short, in the cell line KYSE150 and B16F10, MTA1 play its role to promote cancer cell invasion, adhesion and...