Experimental Research On The Mechanism Of Promoting Blood Circulation To Remove Blood Stasis And Eliminating Heat By Purgation On Acute Cerebralischemia In Rats

Objective: Cerebral ischemia is a neuro-disorder with a high rate of occurrence, mortality, recurrence and disability. Preventive interventions are therefore of great significance as the disease is a serious threat to human health. Although there currently exist neuroprotective agents for acute brain injuries, these therapeutically agents such as NMDA receptor antagonists, calcium channel antagonists, glucocorticoids and other anti-inflammatory drugs tend to produce unsatisfactory results or serious side-effects. In this regard, researches into the neuroprotective properties of Traditional Chinese Medicine (TCM) for acute brain infarction have generated great interests internationally. Based on TCM, brain thrombosis relates to the blood-stasis (Xue Yu Zheng) which can be reverted adequately, as documented clinically, by TCM with anti-blood -stasis property that promotes blood circulation. Li Sheng Capsule has been an effective prescription with years of clinical application. This study combining both TCM and current approaches is to investigate the neuroprotective effects of Li Sheng Capsule on brain injuries in rats, with the aim to reveal the basis of the neuroprotective effects of TCM on Cerebral ischemia in general.Methods: Permanent local ischemia was induced by middle cerebral artery occlusion (MCAO) in rats. After operation, Li Sheng Capsule will give the rats (to give dose immediately according to human mouse equivalent dosage conversion): The model group uses the physiological saline (3ml) to fill the stomach every day; The Chinese native medicine group gives the Li Sheng Capsule 5mg/100g, I/day fills the stomach; the Comparison group gives the brain to wind passes capsule 8mg/100g; Normal Comparison group freely takes in food, potable water. According to pours into the different time is again 6h, 24h, the 3d three time gathering specimen. And through does the wet weight Method to measure the hydro-cephalus change situation; The transmitted light mirror (HE dyes) observes the cerebrum neuron morphology change; With emission immunity method observation blood serum ET , TXB2 and 6-Keto-PGFlα content change; With counter copies the PCRMethod (RT-PCR) to examine the brain to organize VEGF and the bFGF mRNA content;Organizes VEGF...