Study On The Expression Of IL-8 MRNA, MCP-1 MRNA, MIP-1α MRNA And Microvessel Count In Pathological Scars

Background Pathological scars which are common complications among patients suffering from thermal injury, may result in significant function and psychological morbidity. They are elevated tumorous lesions on the skin, firm and erythematous because of the increase in the microvasculture. It has been reported that the degree of hypertrophy after thermal injury correlates with the degree of microvascular regeneration. Also, the fibroblast which highly express collagen gene is found always around new microvessels and concentrate between the lateral branches of the new microvessels. Angiogenesis may play a vital role in the formation of pathological scars. Microvessel count (MVC) of scar tissues could reflect the degree of scar angiogenesis directly.Any tissue has complex angiogenic mechanism modulated by proangiogenic and antiangiogenic factors. Recently it has been found that IL-8, MCP-1 and MCP-1α might play an important role in angiogenesis. Studies substantiated that IL-8, MCP-1 and MIP-1α were important proangiogenic factors. It has been reported recently that fibroblast and endothelial cells could not only secrete IL-8, MCP-1 and MIP-1, but also endothelial cells express their receptors. IL-8, MCP-1 and MIP-1 could promote endothelial cells proliferation and migration by binding to its receptor on endothelial cell surface.Objective To investigate the expressions of IL-8 mRNA, MCP-1 mRNA, MIP-1 α mRNA and their relations with MVC in hypertrophic scars, keloids, normal skin and surgical scar respectively.Methods Immunohistochemical method of avidin-biotin complex was used for MV on the routinely formalin-fixed and paraffin-embedded sections of specimen of hypertrophic scars, keloids, normal skin and...