The Construction Of HBV DNA Oocyte Vector And The Detection Of HBV DNA In The Hepatitis B Patients' Ovaries

Objective To construct HBV DNA oocyte vector and to detect if there is HBV DNA in the hepatitis B patients' ovaries?Methods Molecular biology technique was used to extract mice oocytes, single-cell nested polymerase chain reaction(PCR) was used to detect HBV DNA in oocytes after transfection with PBR322-HBV DNA. Fluorescence in situ hybridization was used to detect HBV DNA in hepatits B patients' ovary paraffin sections.Results â‘ 200 mice oocytes were extracted, of which 180 oocytes were transfected with PBR322-HBV DNA and 20 oocytes weren't transfected. The detection rate of HBV DNA in the mice oocytes after transfection was 69.4% (125/180) , while 0(0/20) in the oocytes without transfection. And the detection rate of HBV DNA in the washing solution of the transfected oocytes was 10%(3/30). The detection rate of HBV DNA in the transfected mice oocytes was higher than the oocytes without transfection(P<0.01), and the washing solution of the transfected oocytes (P<0.01). There was no statistical significance comparing the detection rate of HBV DNA in the mice oocytes without transfection to the washing solution of the transfected oocytes (P>0.05);â‘¡The detection rate of HBV DNA of hepatits B patients' ovary paraffin sections was 70%(14/20), the detection rate of HBV DNA of human ovary paraffin sections with HBsAg(-) was 0(0/20). It was statistically significant comparing the detection rate of HBV DNA of hepatits B patients' ovary paraffin sections to that with HBsAg(-)(P<0.01).Conclusion â‘ Cation liposome can be a kind of good vector to tranfect oocytes with HBV DNA, and oocytes can be the vector of HBV DNA;â‘¡HBV DNA can be found in the hepatits B patients' ovary paraffin sections with HBsAg(+), suggesting that HBV DNA can exist in tissues besides liver.