Microarray Gene Expression Profiles In Heart Failure Resulting From Rheumatic Heart Disease

OBJECTIVES: In this study our goals were (i) to establish whole-genomic gene expression profiles of Chronic Congestive Heart Failure (CHF) resulting from Rheumatic Heart Disease (RHD), (ii) to screen target genes relating to CHF resulting from RHD, (iii) to identify gene clusters relating to CHF caused by RHD and specific genes in each cluster, and (iv) to uncover potential etiologies in the development of the syndrome.METHODS: Firstly, the gene expression profiles of the left ventricular myocardium obtained from cases with CHF resulting from RHD and normal control were profiled by using six human whole-genomic oligonucleotide microarrays (HG U133 Plus 2.0 GeneChip). Between two groups of the fingerprints, GeneSpring software was performed to screen the markedly differentially expressed genes or expressed sequence tags (ESTs) as the target genes relating to CHF resulting from RHD. Secondly, Real-Time PCR was carried out to validate the expression of three target genes. Then, Gene Ontology Analysis and Hierarchical Clustering were performed on target genes by GeneSpring software. Each minor cluster in Hierarchical Clustering was identified as a gene cluster relating to CHF caused by RHD, and specific genes in each cluster were determined. Based on the results, novel potential etiologies in the development of CHF resulting from RHD were...