The Study On The Construction Of HepG2 Cell Expressing Mature Insulin And Glucokinase

Objective:Diabetes is characterized by various kinds of metabolic disorder caused by absolute or relative deficiency of insulin. The etiological factors of diabetes aren't illuminated completely yet. Injecting exogenous insulin, the traditional treatment, often originates correlative complications caused by blood sugar's fluctuation and inappropriate dosage of insulin, because it's not corresponding to physiological mode of insulin secretion. Cell transplantation is the hotspot of recent researches, however, since it's difficult to isolate and store pancreas beta cells and the cost is very expensive, it has been the key problem to find new source of cells for transplantation. This study tries to use gene recombination and transgenic technology, rebuild HepG2 cell to"artificial beta cell"that has the ability of glucose-stimulated insulin secretion (GSIS), and thus find a new way for diabetic cell transplantation.Backgrounds:1,Hepatocyte attends the metabolisms of glucose and lipid directly and has numerous metabolism-related proteins and glucose inducible regulatory elements(GIREs) existing in the genes, so it's the ideal target...