The Pathogenesis Of Cryptorchidism In Mice Induced By Di(2-Ethylhexyl) Phthalate

Objective: to study the correlation between the environmental endocrine disruptor DEHP and the cryptorchidism in mice, to investigate the effect of DEHP on mice testes.Methods: Healthy pregnant KM mice were divided into five groups randomly .Pregnant mice were exposed to DEHP at the dose of 100,200 and 500mg/Kg body weight per day by gavage from gestation day 12(GD12) through postnatal day 3(PND3)(the critical stages of sexual differentiation in mouse)respectively .The corn oil group was given the dissolvent only. After the pregnant mice were killed on GD19,the mice fetuses were removed and were examined under a dissecting microscope. The weight of male pups,sex ratio,the testes-bladder neck relative distances (TBD),location and gross morphology of the testes were recorded. The testes of fetus mice were stained by Hematoxylin and eosin and examined under light microscope. The incidence of cryptorchidism in mice induced by DEHP was investigated.Results:(1) There were no significant alternations in the number of fetuses per pregnant mouse among each group on GD19, but body weight was affected by the DEHP significantly.