Silencing HPV-16 E6 Gene In Human Cervical Cancer CaSki Cell Line By RNA Interference

Cervical cancer is one of the most common malignancy tumor which jejopardize vast female healthy. It indicated that Cervical cancer was highly associated with viral oncogene E6 and E7 of human papillomavirus (HPV). There have been abundant experimental evidences indicating that the E6 and E7oncogenes of HPV play major roles in the development as well as in the maintenance of the malignantphenotype of cervical carcinoma. E6 and E7 of HPV 16,the most frequently associated type of HPV with cervicalcarcinomas, play a role to induce malignant tumor by degradation of the cellular anti-tumor proteins, p53 andretinoblastoma (Rb), respectively in cervical cells. E6 protein forms complexes withp53 and abrogate p53 function of transcriptional activation by stimulating its degradation via ubiquitin -mediated proteolysis in an E6-associated protein (E6AP) mediated reaction, resulting in tumor progression; E7 binds with Rb and cause it phsphorylation and release E2F transcription factor, E2F activates transcription of the genes needed from G1 stage to S stage during cell cycle, resulting in disturbance of cell cycle regulation ,thus make the cell immortalization. Hence, E6 oncogene is one of the ideal targets for directed anti-gene therapy in cervical cancer.RNA interference(RNAi) is a post-transcriptional regulatory manner of gene expression, it is a conservative mode in organism evolution, and it has the effect of counteract invading of virus and maintenance stablity of genome. RNAi could knockdown the expression of gene in mammalians, which...