Foundation Exploratory Development Of Hepatitis C Virus And Borna Disease Virus Infection In Viral Encephalitis

Objective:This study aimed to establish a fast and accurate quantitative method to detect hepatitis C virus(HCV)and Borna disease virus(BDV)ribonucleic acid(RNA)in peripheral blood(PB)and cerebrospinal fluid(CSF)using fluorescence quantitative nested reverse transcriptase polymerase chain reaction(FQ-nRT-PCR),and to investigate the relationship between HCV or BDV infection and the Viral encephalitis(VE)and sheep. Further,the gene sequence and amino acid sequence for BDV positive product were analyzed to establish the molecular epidemiologic characteristic by drawing phylogenetic tress.Methods:1.According to the specific sequence of HCV E1 gene,the primers and the fluorescence probe were synthesized as designed.The fragments amplified by PCR were cloned into the pMD18-T vector.The positive recombinant plasmid would be used as standard quantitative template to make the standard curve for sample detection,so as to establish a method of...