The Effect Of RelB Gene Silencing On The Bioimmunological Function Of Bone Marrow Dendritic Cells

Objective: Recent researches showed that dendritic cells can either initiate T cell immunoreaction or promote peripheral tolerance depending on their state of maturaton. Tolerogenic dendritic cells(Tol-DC) have been defined by immature phenotype and the ability to inhibit T cell responses. RelB is a critical nuclear transcription factor NF- κB family member required for myeloid DC maturation. RelB gene silencing maybe endows DC with tolerogenic ability through blockade of NF- κB activity. Our research group schedules to establish the culture method to proliferate highly pure bone marrow-derived dendritic cells from mice, identificate the effective siRNA against RelB gene by siRNA expression cassett, finally construct a new type of DC—RNAi RelB DC. The RNAi RelB DC will be studied in vitro on cell morphalogy, cell phenotype, cell immunofunction to evaluate its tolerogenic ability and the possible application in treatment of organ transplant rejection and some autoimmune diseases.Methods:1, Culture and identification of bone marrow-derived dendritic cellsDendritic cells were generated from C57BL/6 mice by culturing bone marrow precursors with recombinant mouse granulocyte-marophage colony-stimulating factor and interlukin-4 (rmGM-CSF, rmIL-4) using handful isolation combined...