Application Of Microarray In Study On Apoptotic Mechanism Of HeLa Cells Induced By HPV18 E6 SiRNA And HPV Detection

Cervical cancer is one of the most common malignancies and preceded only by breast cancer as the second leading cause of cancer mortality in women worldwide. Nearly 400,000 cases were diagnosed and over 200,000 women were killed annually worldwide, 80% of which occurred in the developing countries. During the past 20 years, the link between genital Human papillomavirus (HPV) infections and cervical cancer have been identified.HPVs are small, double-stranded DNA virus which specifically infect epithelial tissues and induce hyperproliferative lesions. The HPV genome contains 8,000bp and is functionally divided into three regions: (i) Early region of 4,500bp, consisting of ORFs El, E2, E4, E5, E6, and E7, which are involved in viral DNA replication, transcription and cell transformation, (ii) Late region of 2,500bp, which encodes the L1 and L2 structural proteins for the viral capsid. (iii) Noncoding upstream regulatory region (URR) or the long control region (LCR) of 400 to l,000bp, located between the E region and L region. This region contains promoter along with enhancer and...