Molecular Basis Study On Inhibition Of Erigeron Breviscapus Element On The Proliferation Of Vascular Smooth Muscle Cell

Objective The aim of the study is to evaluate effects of antisense thrombin receptor oligo-deoxy-nucleotides (TR ODNs) and Erigeron breviscapus element in proliferation of Vascular Smooth Muscle Cell, which is induced by thrombin. Comparing and analyzing the Vascular Smooth Muscle Cell thrombin receptor expression, which were treated with antisense TR ODNs and Erigeron breviscapus element are to explore the mechanism of action of preventive dose and drug screening in hypertensive cerebral hemorrhage at molecular level.Methods Applying cell transfection, RT-PCR Western-Blot, E1ISA, ~3H-TDR and ~3H inositol unincorporation etc, to assay the thrombin receptor gene and protein expression, which was cultured by the fourth-sixth generation Sprague-Dawley rat (SD)'s Vascular Smooth Muscle Cell in vitro. To explore the influence of Erigeron breviscapus element and antisense thrombin receptor oligo-deoxy-nucleotides on the proliferation of Vascular Smooth Muscle Cell.Results Erigeron breviscapus element and antisense thrombin can inhibit the proliferation of SD's Vascular Smooth Muscle Cell, which is induced by thrombin. Comparing the obverse with control group, the statistic difference still exists.(p<0.05) The experimental result of RT-PCR, Western-Blot and E1ISA indicates that Erigeron breviscapus element and antisense thrombin restrains the expression of mRNA and protein in SD's Vascular Smooth Muscle Cell distinctly, and inhibits phosphoric inositol metabolism of Vascular Smooth Muscle Cell distinctly as well. There is no difference in inhabitation effects between Erigeron breviscapus element and atisense...