Association Analysis Of The Parkin Gene And Mutation Screening In Sporadic Parkinson's Disease Patients And Family Parkinsonism Patients

Objective The aims of the study were to determine: whether there were any associations between the Serl67Asn polymorphism, the -258T / G polymorphism of the promoter and the IVS3-20Tâ–¡C polymorphism in Parkin gene and Parkinson's disease from a Han population in Sichuan province, whether there were homozygous deletions or point mutations in the Parkin gene in early-onset patients with PD and family PD patients from Sichuan province.Materials and method Polymerase chain reaction(PCR), denaturing high performance liquid chromatography(dHPLC)and sequence analysis were used to determine the genotype of each subject. We analysed the known common Ser167Asn polymorphism in 116 patients, the -258T/G polymorphism and IVS3-20Tâ–¡C polymorphism in 198 patients with sporadic PD and controls, matched for age and gender. Parkin gene mutations were detected by using...